quick ligase reaction buffer Search Results


1x t4 rna ligase reaction buffer  (New England Biolabs)
96
New England Biolabs 1x t4 rna ligase reaction buffer
1x T4 Rna Ligase Reaction Buffer, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/1x t4 rna ligase reaction buffer/product/New England Biolabs
Average 96 stars, based on 1 article reviews
1x t4 rna ligase reaction buffer - by Bioz Stars, 2026-05
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1x neb t4 dna ligase buffer  (New England Biolabs)
97
New England Biolabs 1x neb t4 dna ligase buffer
1x Neb T4 Dna Ligase Buffer, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/1x neb t4 dna ligase buffer/product/New England Biolabs
Average 97 stars, based on 1 article reviews
1x neb t4 dna ligase buffer - by Bioz Stars, 2026-05
97/100 stars
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t4 ligase buffer  (New England Biolabs)
97
New England Biolabs t4 ligase buffer
T4 Ligase Buffer, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/t4 ligase buffer/product/New England Biolabs
Average 97 stars, based on 1 article reviews
t4 ligase buffer - by Bioz Stars, 2026-05
97/100 stars
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taq dna ligase reaction buffer  (New England Biolabs)
94
New England Biolabs taq dna ligase reaction buffer
Argonaute endonuclease reactions were performed in <t>Taq</t> <t>DNA</t> <t>Ligase</t> Reaction Buffer at 74 °C for 2 hr. Upon completion, the disappearance of substrate was monitored by CE, and the reactions were subsequently split and treated with either Taq or T4 DNA ligase supplemented with NAD + or ATP, respectively. Full-length product was then re-annealed, ligated, and detected by CE—fluorescence peak data is shown above each substrate/product diagram. ( A ) The TAMRA dye is attached to the synthetic oligo via a modified thymidine; as such, the two targets differ in sequence by one base—a T in the 3′ terminal position of the TAMRA-labeled oligo becomes a G in the HEX-labeled oligo. This causes a slight difference in capillary migration time for the two FAM-labeled products. ( B ) Fluorophore hydrophobicity also affects product migration in the capillary; therefore, the peaks displayed correlate with the size as determined by a standard, which slightly differs from the actual length of the product. ( C ) T4 DNA ligase is able to generate a broader palate of ligation products than Taq DNA ligase due to increased functionality at lower temperatures and the ability to perform blunt end ligation. Peaks marked with (*) arise from ligation events involving excess 5′-phosphorylated DNA guide.
Taq Dna Ligase Reaction Buffer, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/taq dna ligase reaction buffer/product/New England Biolabs
Average 94 stars, based on 1 article reviews
taq dna ligase reaction buffer - by Bioz Stars, 2026-05
94/100 stars
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buffer  (New England Biolabs)
94
New England Biolabs buffer
Argonaute endonuclease reactions were performed in <t>Taq</t> <t>DNA</t> <t>Ligase</t> Reaction Buffer at 74 °C for 2 hr. Upon completion, the disappearance of substrate was monitored by CE, and the reactions were subsequently split and treated with either Taq or T4 DNA ligase supplemented with NAD + or ATP, respectively. Full-length product was then re-annealed, ligated, and detected by CE—fluorescence peak data is shown above each substrate/product diagram. ( A ) The TAMRA dye is attached to the synthetic oligo via a modified thymidine; as such, the two targets differ in sequence by one base—a T in the 3′ terminal position of the TAMRA-labeled oligo becomes a G in the HEX-labeled oligo. This causes a slight difference in capillary migration time for the two FAM-labeled products. ( B ) Fluorophore hydrophobicity also affects product migration in the capillary; therefore, the peaks displayed correlate with the size as determined by a standard, which slightly differs from the actual length of the product. ( C ) T4 DNA ligase is able to generate a broader palate of ligation products than Taq DNA ligase due to increased functionality at lower temperatures and the ability to perform blunt end ligation. Peaks marked with (*) arise from ligation events involving excess 5′-phosphorylated DNA guide.
Buffer, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/buffer/product/New England Biolabs
Average 94 stars, based on 1 article reviews
buffer - by Bioz Stars, 2026-05
94/100 stars
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ligase reaction buffer  (Promega)
90
Promega ligase reaction buffer
Argonaute endonuclease reactions were performed in <t>Taq</t> <t>DNA</t> <t>Ligase</t> Reaction Buffer at 74 °C for 2 hr. Upon completion, the disappearance of substrate was monitored by CE, and the reactions were subsequently split and treated with either Taq or T4 DNA ligase supplemented with NAD + or ATP, respectively. Full-length product was then re-annealed, ligated, and detected by CE—fluorescence peak data is shown above each substrate/product diagram. ( A ) The TAMRA dye is attached to the synthetic oligo via a modified thymidine; as such, the two targets differ in sequence by one base—a T in the 3′ terminal position of the TAMRA-labeled oligo becomes a G in the HEX-labeled oligo. This causes a slight difference in capillary migration time for the two FAM-labeled products. ( B ) Fluorophore hydrophobicity also affects product migration in the capillary; therefore, the peaks displayed correlate with the size as determined by a standard, which slightly differs from the actual length of the product. ( C ) T4 DNA ligase is able to generate a broader palate of ligation products than Taq DNA ligase due to increased functionality at lower temperatures and the ability to perform blunt end ligation. Peaks marked with (*) arise from ligation events involving excess 5′-phosphorylated DNA guide.
Ligase Reaction Buffer, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/ligase reaction buffer/product/Promega
Average 90 stars, based on 1 article reviews
ligase reaction buffer - by Bioz Stars, 2026-05
90/100 stars
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e3 ligase buffer  (Boston Biochem)
90
Boston Biochem e3 ligase buffer
Argonaute endonuclease reactions were performed in <t>Taq</t> <t>DNA</t> <t>Ligase</t> Reaction Buffer at 74 °C for 2 hr. Upon completion, the disappearance of substrate was monitored by CE, and the reactions were subsequently split and treated with either Taq or T4 DNA ligase supplemented with NAD + or ATP, respectively. Full-length product was then re-annealed, ligated, and detected by CE—fluorescence peak data is shown above each substrate/product diagram. ( A ) The TAMRA dye is attached to the synthetic oligo via a modified thymidine; as such, the two targets differ in sequence by one base—a T in the 3′ terminal position of the TAMRA-labeled oligo becomes a G in the HEX-labeled oligo. This causes a slight difference in capillary migration time for the two FAM-labeled products. ( B ) Fluorophore hydrophobicity also affects product migration in the capillary; therefore, the peaks displayed correlate with the size as determined by a standard, which slightly differs from the actual length of the product. ( C ) T4 DNA ligase is able to generate a broader palate of ligation products than Taq DNA ligase due to increased functionality at lower temperatures and the ability to perform blunt end ligation. Peaks marked with (*) arise from ligation events involving excess 5′-phosphorylated DNA guide.
E3 Ligase Buffer, supplied by Boston Biochem, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/e3 ligase buffer/product/Boston Biochem
Average 90 stars, based on 1 article reviews
e3 ligase buffer - by Bioz Stars, 2026-05
90/100 stars
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quick ligasing buffer  (Promega)
90
Promega quick ligasing buffer
Argonaute endonuclease reactions were performed in <t>Taq</t> <t>DNA</t> <t>Ligase</t> Reaction Buffer at 74 °C for 2 hr. Upon completion, the disappearance of substrate was monitored by CE, and the reactions were subsequently split and treated with either Taq or T4 DNA ligase supplemented with NAD + or ATP, respectively. Full-length product was then re-annealed, ligated, and detected by CE—fluorescence peak data is shown above each substrate/product diagram. ( A ) The TAMRA dye is attached to the synthetic oligo via a modified thymidine; as such, the two targets differ in sequence by one base—a T in the 3′ terminal position of the TAMRA-labeled oligo becomes a G in the HEX-labeled oligo. This causes a slight difference in capillary migration time for the two FAM-labeled products. ( B ) Fluorophore hydrophobicity also affects product migration in the capillary; therefore, the peaks displayed correlate with the size as determined by a standard, which slightly differs from the actual length of the product. ( C ) T4 DNA ligase is able to generate a broader palate of ligation products than Taq DNA ligase due to increased functionality at lower temperatures and the ability to perform blunt end ligation. Peaks marked with (*) arise from ligation events involving excess 5′-phosphorylated DNA guide.
Quick Ligasing Buffer, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/quick ligasing buffer/product/Promega
Average 90 stars, based on 1 article reviews
quick ligasing buffer - by Bioz Stars, 2026-05
90/100 stars
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10x t4 dna ligase reaction buffer  (ABclonal)
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10x e.coli dna ligase reaction buffer  (ABclonal)
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10x taq dna ligase reaction buffer  (ABclonal)
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Image Search Results


Argonaute endonuclease reactions were performed in Taq DNA Ligase Reaction Buffer at 74 °C for 2 hr. Upon completion, the disappearance of substrate was monitored by CE, and the reactions were subsequently split and treated with either Taq or T4 DNA ligase supplemented with NAD + or ATP, respectively. Full-length product was then re-annealed, ligated, and detected by CE—fluorescence peak data is shown above each substrate/product diagram. ( A ) The TAMRA dye is attached to the synthetic oligo via a modified thymidine; as such, the two targets differ in sequence by one base—a T in the 3′ terminal position of the TAMRA-labeled oligo becomes a G in the HEX-labeled oligo. This causes a slight difference in capillary migration time for the two FAM-labeled products. ( B ) Fluorophore hydrophobicity also affects product migration in the capillary; therefore, the peaks displayed correlate with the size as determined by a standard, which slightly differs from the actual length of the product. ( C ) T4 DNA ligase is able to generate a broader palate of ligation products than Taq DNA ligase due to increased functionality at lower temperatures and the ability to perform blunt end ligation. Peaks marked with (*) arise from ligation events involving excess 5′-phosphorylated DNA guide.

Journal: PLoS ONE

Article Title: Single-stranded binding proteins and helicase enhance the activity of prokaryotic argonautes in vitro

doi: 10.1371/journal.pone.0203073

Figure Lengend Snippet: Argonaute endonuclease reactions were performed in Taq DNA Ligase Reaction Buffer at 74 °C for 2 hr. Upon completion, the disappearance of substrate was monitored by CE, and the reactions were subsequently split and treated with either Taq or T4 DNA ligase supplemented with NAD + or ATP, respectively. Full-length product was then re-annealed, ligated, and detected by CE—fluorescence peak data is shown above each substrate/product diagram. ( A ) The TAMRA dye is attached to the synthetic oligo via a modified thymidine; as such, the two targets differ in sequence by one base—a T in the 3′ terminal position of the TAMRA-labeled oligo becomes a G in the HEX-labeled oligo. This causes a slight difference in capillary migration time for the two FAM-labeled products. ( B ) Fluorophore hydrophobicity also affects product migration in the capillary; therefore, the peaks displayed correlate with the size as determined by a standard, which slightly differs from the actual length of the product. ( C ) T4 DNA ligase is able to generate a broader palate of ligation products than Taq DNA ligase due to increased functionality at lower temperatures and the ability to perform blunt end ligation. Peaks marked with (*) arise from ligation events involving excess 5′-phosphorylated DNA guide.

Article Snippet: The pMiniT™ 2.0 vector; pUC19 vector; pBR322 vector; ΦX174 Virion, RFI, and RFII DNA; Extreme-Thermostable Single-Stranded DNA Binding Protein (ET SSB); T4 Gene 32 Protein (gp32); Proteinase K; SspI-HF ® ; Nt.BspQI; ThermoPol ® Reaction Buffer (20 mM Tris(hydroxymethyl)aminomethane hydrochloride (Tris-HCl) pH 8.8 @ 25 °C, 10 mM potassium chloride, 10 mM ammonium sulfate, 2 mM magnesium sulfate, and 0.1% Triton X-100); Taq DNA Ligase Reaction Buffer (20 mM Tris-HCl pH 7.6 @ 25 °C, 25 mM potassium acetate, 10 mM magnesium acetate, 1 mM NAD + , 10 mM dithiothreitol (DTT), and 0.1% Triton X-100); NEBuffer™ 3.1; NEBuffer™ 4; CutSmart ® Buffer; Diluent E; and Quick-Load Purple 2-Log and Low Molecular Weight (LMW) DNA Ladders were obtained from New England Biolabs, Inc. (NEB; Ipswich, Massachusetts, USA).

Techniques: Fluorescence, Modification, Sequencing, Labeling, Migration, Ligation